Isolation and 13C‐NMR characterization of an insoluble proteinaceous fraction from substantia nigra of patients with parkinson's disease
Identifieur interne : 001C15 ( Main/Exploration ); précédent : 001C14; suivant : 001C16Isolation and 13C‐NMR characterization of an insoluble proteinaceous fraction from substantia nigra of patients with parkinson's disease
Auteurs : Silvio Aime [Italie] ; Bruno Bergamasco [Italie] ; Mariano Casu [Italie] ; Giuseppe Digilio [Italie] ; Mauro Fasano [Italie] ; Sabrina Giraudo [Italie] ; Leonardo Lopiano [Italie]Source :
- Movement Disorders [ 0885-3185 ] ; 2000-09.
English descriptors
Abstract
Neuromelanin is a dark brown pigment suspected of being involved in the pathogenesis of Parkinson's disease. This pigment can be isolated from normal human substantia nigra by a procedure that includes an extensive proteolytic treatment. In this study we used such a procedure to extract the neuromelanin pigment from a pool of substantia nigra from patients affected by Parkinson's disease. 13C Cross polarization magic angle spinning nuclear magnetic resonance spectroscopy and electron paramagnetic resonance spectroscopy were used to characterize the solid residue obtained from the extraction procedure. We found that the pigment extracted from the substantia nigra of parkinsonian patients was mainly composed of highly cross‐linked, protease‐resistant, lipo‐proteic material, whereas the neuromelanin macromolecule appears to be only a minor component of this extract. A synthetic model of melanoprotein has been prepared by enzymatic oxidation of dopamine in the presence of albumin. Once it has undergone the same proteolytic treatment, this model system yields a 13C‐NMR spectrum which is similar to that observed for the parkinsonian midbrain extract. These results are consistent with the view that oxidative stress has a relevant role in the pathogenesis of Parkinson's disease.
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DOI: 10.1002/1531-8257(200009)15:5<977::AID-MDS1032>3.0.CO;2-Q
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nuts="2">Piémont</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Movement Disorders</title><title level="j" type="abbrev">Mov. 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This pigment can be isolated from normal human substantia nigra by a procedure that includes an extensive proteolytic treatment. In this study we used such a procedure to extract the neuromelanin pigment from a pool of substantia nigra from patients affected by Parkinson's disease. 13C Cross polarization magic angle spinning nuclear magnetic resonance spectroscopy and electron paramagnetic resonance spectroscopy were used to characterize the solid residue obtained from the extraction procedure. We found that the pigment extracted from the substantia nigra of parkinsonian patients was mainly composed of highly cross‐linked, protease‐resistant, lipo‐proteic material, whereas the neuromelanin macromolecule appears to be only a minor component of this extract. A synthetic model of melanoprotein has been prepared by enzymatic oxidation of dopamine in the presence of albumin. Once it has undergone the same proteolytic treatment, this model system yields a 13C‐NMR spectrum which is similar to that observed for the parkinsonian midbrain extract. These results are consistent with the view that oxidative stress has a relevant role in the pathogenesis of Parkinson's disease.</div></front></TEI><affiliations><list><country><li>Italie</li></country><region><li>Piémont</li></region><settlement><li>Turin</li></settlement></list><tree><country name="Italie"><region name="Piémont"><name sortKey="Aime, Silvio" sort="Aime, Silvio" uniqKey="Aime S" first="Silvio" last="Aime">Silvio Aime</name></region><name sortKey="Bergamasco, Bruno" sort="Bergamasco, Bruno" uniqKey="Bergamasco B" first="Bruno" last="Bergamasco">Bruno Bergamasco</name><name sortKey="Casu, Mariano" sort="Casu, Mariano" uniqKey="Casu M" first="Mariano" last="Casu">Mariano Casu</name><name sortKey="Digilio, Giuseppe" sort="Digilio, Giuseppe" uniqKey="Digilio G" first="Giuseppe" last="Digilio">Giuseppe Digilio</name><name sortKey="Fasano, Mauro" sort="Fasano, Mauro" uniqKey="Fasano M" first="Mauro" last="Fasano">Mauro Fasano</name><name sortKey="Giraudo, Sabrina" sort="Giraudo, Sabrina" uniqKey="Giraudo S" first="Sabrina" last="Giraudo">Sabrina Giraudo</name><name sortKey="Lopiano, Leonardo" sort="Lopiano, Leonardo" uniqKey="Lopiano L" first="Leonardo" last="Lopiano">Leonardo Lopiano</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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